Studies often use siCHMP4 to monitor the localization of fluorescently tagged proteins (e.g., eGFP-tagged CHMP4B) to confirm that the protein is gone or to observe how other proteins react to its absence [5.4].
CHMP4 is the main component of the ESCRT-III complex [5.3]. Sichmp4
Details on the experimental design of a siCHMP4 experiment Studies often use siCHMP4 to monitor the localization
While initial membrane bending might be driven by protein crowding (an energy-independent process), the final constriction and scission driven by CHMP4/ESCRT-III filaments require the ATPase VPS4 [5.3, 5.1]. Consequences of siCHMP4 (Knockdown) 5.1]. When cells are treated with
Together with other components, CHMP4 forms filaments that drive the constriction and scission of membrane necks [5.3, 5.1].
When cells are treated with , the following phenotypes are commonly observed: