High percentages (usually >70%) indicate high-quality data and successful alignment.
The most direct way to obtain STAR is via its official GitHub repository. Download STAR bin
To download and use the (Spliced Transcripts Alignment to a Reference) binaries to generate a report, follow the steps below. This report is commonly used in bioinformatics to align RNA-seq reads to a reference genome. 1. Download STAR Binaries Download STAR bin
STAR --runThreadN [threads] \ --runMode genomeGenerate \ --genomeDir /path/to/index/ \ --genomeFastaFiles /path/to/genome.fasta \ --sjdbGTFfile /path/to/annotation.gtf Use code with caution. 3. Run Alignment and Generate Report Download STAR bin