Are you using this for (like at Addgene)?
What is the of the content you want to create (e.g., guide, report, project description)?
It allows researchers to insert a specific 20-nucleotide guide sequence to target Cas9 to a specific genomic location. Vector Type: Mammalian Expression Vector. Key Components: U6 Promoter: Drives the high-level expression of the gRNA. 47108.rar
Note: The search results also indicate this is part of a larger, often complex data set.
To help me provide more relevant content, could you tell me: Are you using this for (like at Addgene)
Explain cloning a target sequence into the BbsI sites of pSPgRNA.
pSPgRNA is a plasmid designed for the expression of a guide RNA (gRNA) for CRISPR-Cas9 systems. 47108.rar
Expression of the chimeric guide RNA in mammalian cells.